Characterization of Soybean Polyuquitin Promoters
via Transgenic
Expression Assay
- Ubiquitin (originally, ubiquitous immunopoietic polypeptide) was first identified in 1975 as an 8.5-kDa protein of unknown function expressed universally in living cells. The basic functions of ubiquitin and the components of the ubiquitination pathway were elucidated in the early 1980s in groundbreaking work performed at Fox Chase Cancer Center by Aaron Ciechanover, Avram Hershko, and Irwin Rose for which the Nobel Prize in Chemistry was awarded in 2004.
- The polyubiquitin (Ubi) gene family in soybean contains 3 moderately well characterized genes
(Ubi1, 2, 3) as well as other family members that have received little to no attention. The promoters regulating these genes have likewise not been well characterized but show
promise as strong constitutive promoters, based on gene expression data.
- We are performing extensive characterization of these Gmubi promoters such as Glycine max ubiquitin3
(Gmubi3) in transgenic plants. Promoters from polyubiquitin genes containing tetrameric repeats
of 76 amino acids were selected,
all of the promoters (~1.4 kb) were PCR-amplified from genomic DNA of cultivar Jack. We are testing soybean Ubi promoters isolated from JGI soybean genomic assembles with state-of-the-art
.
- There are at least 73
in soybean, we are currently isolating and characterizing
the following 10 Ubi promoters for expression characterization
|
Ubi Promoters |
|
Promoter Size (bp) |
| Gmubi1 |
Glyma10g39780 |
1,449 |
| Gmubi2 |
Glyma13g17830.1 |
1,484 |
| Gmubi3 |
Glyma20g27950.1 |
1,438 |
| Gmubi4 |
Glyma13g24470.1 |
1,430 |
| Gmubi5 |
Glyma13g24500.1 |
1,452 |
| Gmubi6 |
Glyma07g32020.1 |
1,409 |
| Gmubi7 |
Glyma17g04690.1 |
1,453 |
| Gmubi8 |
Glyma10g05830.1 |
892 |
| Gmubi9 |
Glyma13g20200 |
1,314 |
| Gmubi10 |
Glyma15g13650.1 |
1,355 |
- Our results for the above mentioned 10 promoters with graphic transient expression
intensities in lima bean cotyledons and soybean hairy roots.
- A video tape movie can be downloaded on the dynamic expression patterns of the tested
Ubi promoters, which can be played with
QuickTime Player. As the movie file is large (about 40 MB), when the link is clicked, it takes about
10-20 min for both the latest Microsoft IE and Firefox to play the movie within the web browsers.
This project is led by Dr. John Finer at
Ohio State University and funded by
:
.
Last updated with the site by Dr. Jeff Chen on September 8,
2009.
