EIL Transcription Factor Family
ETHYLENE INSENSITIVE3 (EIN3) are transcription factors involved in the ethylene signal transduction pathway in Arabidopsis
and higher plants. Two full-length cDNA clones, pVR-EIL1 and pVR-EIL2, encoding EIN3-LIKE proteins were isolated by reverse
transcriptase-polymerase chain reaction and by screening the cDNA library of mung bean (Vigna radiata) hypocotyls. VR-EIL1
and VR-EIL2 share 70% identity and display varying degrees of sequence conservation (39%¨C65%) with previously isolated EIN3
homologs from Arabidopsis, tobacco (Nicotiana tabacum) and tomato (Lycopersicon esculentum) plants. Gel retardation assay
revealed that both VR-EILs were able to interact specifically with optimal binding sequence-1, the recently identified
optimal binding sequence for tobacco TEIL, with the binding of VR-EIL2 being more efficient than that of VR-EIL1. Transient
expression analysis using a VR-EIL::smGFP fusion gene in onion (Allium cepa) epidermal cells indicated that the VR-EIL
proteins were effectively targeted to the nucleus. The fusion protein of VR-EIL2 with GAL4 DNA-binding domain strongly
activated transcription of a reporter gene in yeast cells, and an essential domain for transcription-stimulating activity
was localized to the amino-terminal acidic region that consists of 50 amino acid residues. In contrast with what has been
previously found in EIN3- and TEIL-overexpressing Arabidopsis plants, transgenic tobacco seedlings expressing the VR-EIL
genes under the control of cauliflower mosaic virus 35S promoter did not exhibit a constitutive triple response.
14 predicted putative EIL TF peptide,
CDS, and cDNA sequences;
blast HSP, and gene level multiple sequence alignment in
Soy - TFKB.
EIN3 domain peptide sequences and alignment.
12 EIL protein
and DNA
sequences with
annotations for soybean in PlantTFDB. Most are partial sequences.
Last updated by Dr. Jeff Chen
on June 5, 2009.