LIM Transcription Factor Family
The AC-rich motif, Pal-box, is an important cis-acting element for gene expression involved in phenylpropanoid biosynthesis.
A cDNA clone (Ntlim1) encoding a Pal-box binding protein was isolated by Southwestern screening. The deduced amino acid
sequence is highly similar to the members of the LIM protein family that contain a zinc finger motif. Moreover, Ntlim1 had a
specific DNA binding ability and transiently activated the transcription of a beta-glucuronidase reporter gene driven by the
Pal-box sequence in tobacco protoplasts. The transgenic tobacco plants with antisense Ntlim1 showed low levels of
transcripts from some key phenylpropanoid pathway genes such as phenylalanine ammonia-lyase, hydroxycinnamate CoA ligase and
cinnamyl alcohol dehydrogenase. Furthermore, a 27% reduction of lignin content was observed in the transgenic tobacco with
antisense Ntlim1.
26 predicted LIM TF peptide,
CDS, and cDNA sequences;
blast HSP, and multiple sequence alignment.
22 LIM domain peptide sequences with alignment
and domain-based phylogeny tree.
25 LIM protein
and DNA
sequences with
annotations for soybean in PlantTFDB. Most are partial sequences.
Last updated by Dr. Jeff Chen on
June 15, 2009.
