SRS Transcription Factor Family
SHI RELATED SEQUENCE(SRS), which encode proteins with a single zinc finger motif. The genes are
members of a small gene family of putative transcription factors in which the SHORT INTERNODES
(SHI) gene is found.
The predicted amino acid sequence of SHI has acidic and glutamine-rich stretches and shows
sequence similarity over a putative zinc finger region to three presumptive Arabidopsis
proteins. This suggests that SHI may act as a negative regulator of GA responses through
transcriptional control.
The predicted SHI protein consists of 331 amino acids. A region containing six cysteine residues
(amino acids 120 to 147) in a C-X2-C-X7-C-X4-C-X2-C-X7-C arrangement (where X is a variable
amino acid) is suggested to make up a zinc finger domain similar to the Zn2Cys6 cluster found in
the DNA binding region of the yeast transcriptional activator GAL4 (Kraulis et al. 1992). Two
regions rich in glutamine residues were found adjacent to the zinc finger domain, as well as a
short stretch of acidic residues in the C-terminal third of the SHI protein. These features have
been found in transcription factor proteins and have been demonstrated to be important for their
function as transcriptional activators (Mitchell and Tjian 1989). Furthermore, two putative
nuclear localization signals (NLSs) were found in the SHI sequence. These are two basic
stretches (amino acids 160 to 165 and 183 to 188) that conform to the consensus of the simian
virus 40¨Clike "typical NLS" defined as four arginines and lysines within a region of six amino
acids (Boulikas 1994 ; LaCasse and Lefebvre 1995).
24 predicated SRS TF peptide,
CDS, and cDNA sequences;
blast HSP, multiple sequence alignment,
and phylogeny tree in
Soy - TFKB.
5 SRS protein
and DNA
sequences with
annotations for soybean in PlantTFDB. Most arepartial sequences.
Last updated by Dr. Jeff Chen on July 20, 2009.
