VOZ Transcription Factor Family
A 38-bp pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis thaliana V-PPase
during pollen development. Here, we report the isolation and structural characterization of AtVOZ1 and AtVOZ2, novel
transcription factors that bind to the 38-bp cis-acting region of A. thaliana V-PPase gene, AVP1. AtVOZ1 and AtVOZ2 show 53%
amino acid sequence similarity. Homologs of AtVOZ1 and AtVOZ2 are found in various vascular plants as well as a moss,
Physcomitrella patens. Promoter-beta-glucuronidase reporter analysis shows that AtVOZ1 is specifically expressed in the
phloem tissue and AtVOZ2 is strongly expressed in the root. In vivo transient effector-reporter analysis in A. thaliana
suspension-cultured cells demonstrates that AtVOZ1 and AtVOZ2 function as transcriptional activators in the Arabidopsis
cell. Two conserved regions termed Domain-A and Domain-B were identified from an alignment of AtVOZ proteins and their
homologs of O. sativa and P. patens. AtVOZ2 binds as a dimer to the specific palindromic sequence, GCGTNx7ACGC, with
Domain-B, which is comprised of a functional novel zinc coordinating motif and a conserved basic region. Domain-B is shown
to function as both the DNA-binding and the dimerization domains of AtVOZ2. From highly the conservative nature among all
identified VOZ proteins, we conclude that Domain-B is responsible for the DNA binding and dimerization of all VOZ-family
proteins and designate it as the VOZ-domain.
(taken from TOBFAC).
Notes: VOZ has 9 genes in AtTFDB with protein protein interaction data.
8 VOZ sequences,
blast HSP, and multiple sequence alignment in
Legume TFKB. Some are full length proteins and
CDS.
8 VOZ domain peptide sequences with alignment via domain based search.
3 VOZ sequences in PlantTFDB. All partial sequences.
Last updated by Dr. Jeff Chen on May 23, 2009.